Manipulating the Mouse Genome Using Recombineering
نویسندگان
چکیده
منابع مشابه
Manipulating the Mouse Genome Using Recombineering
Animal models are routinely used to understand the etiology of human genetic disorders, as well as to develop preclinical models to test the efficacy of novel therapies. Although there are speciesspecific variations based on gene function studies, the mouse is the most reliable and commonly used model system, mainly because of its genetic and physiological similarity to humans. In addition, adv...
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In 1974, Dr. Ralph Brinster published a paper describing the consequences of injecting embryonal carcinoma cells, the predecessors of embryonic stem cells, into mouse blastocysts. Despite their early promise, embryonal carcinoma cells would not efficiently populate the germ line of mice. A decade later Elizabeth Robertson and I described the efficient generation of germline chimaeras from cultu...
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Genome manipulation, the primary tool for assigning function to sequence, will be essential for understanding Plasmodium biology and malaria pathogenesis in molecular terms. The first success in transfecting Plasmodium was reported almost ten years ago. Gene-targeting studies have since flourished, as Plasmodium is haploid and integrates DNA only by homologous recombination. These studies have ...
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ژورنال
عنوان ژورنال: Advancements in Genetic Engineering
سال: 2013
ISSN: 2169-0111
DOI: 10.4172/2169-0111.1000108